This study evaluated the microbial and physicochemical quality of street-vended fruit juices sold in selected barangays of Sagay City and determined their safety for public consumption. Microbial analysis focused on the detection of coliforms, total coliforms, fecal coliforms, and Escherichia coli using the Most Probable Number (MPN) standard. Physicochemical parameters analyzed included total soluble solids (TSS), pH, total dissolved solids (TDS), fat, protein, carbohydrate content, and total acidity expressed as citric acid. Samples were collected from various street vendors across the city. Physicochemical analyses were conducted at the Negros Prawn Producers Cooperative Analytical and Diagnostic Laboratory (NPPC), while microbial examinations were performed at the NONESCOST laboratory using the standard multiple-tube fermentation technique based on the Philippine National Standards for Drinking Water. The findings revealed elevated levels of coliform contamination in fruit juice samples obtained from Barangay Old Sagay, Barangay Poblacion 2, and Barangay Paraiso. The presence of high coliform counts indicates possible fecal contamination and suggests that the juices do not meet potable safety standards, posing potential health risks to consumers. In contrast, the physicochemical characteristics of the samples were found to be within acceptable limits and complied with established standards for safe consumption. These results indicate that although the nutritional and chemical properties of the fruit juices were satisfactory, microbial contamination remains a significant concern. The study highlights the need for regular monitoring, stricter sanitary practices, and the implementation of standardized assessment protocols for street-vended fruit juices. Furthermore, the findings serve as valuable information for public health authorities and consumers, particularly tourists, in promoting food safety awareness and reducing the risk of foodborne illnesses.